Description
The NucleoMag RNA Blood Kit is intended for the purification of RNA from whole blood samples collected in EDTA / Citrate blood collection tubes or in SARSTEDT® S-Monovette RNA Exact, Zymo DNA/RNA Shield™ Blood Tubes, or Tempus™ RNA Blood Tubes. With a focus on simplicity and efficiency, this kit eliminates the need for reagent removal and pelleting*, ensuring smooth processing. The purified RNA obtained from this kit is suitable for a range of downstream applications including RT-PCR and sequencing. The kit can be used either manually or automated on standard liquid handling instruments or automated magnetic separators. Included in the kit are essential components such as NucleoMag B-Beads, Buffers, Liquid Proteinase K, and RNase-free rDNase, ensuring reliable and high-quality RNA extraction. Note: Blood collection and stabilization tubes are not provided and must be purchased separately. *Processing of EDTA / Citrate or Tempus RNA Blood Tubes necessitates additional equipment and buffers as detailed in the user manual for comprehensive guidance.
| Application | Isolation of RNA from whole blood |
| Selling unit | 96 Prep(s), 384 Prep(s) |
| Target | RNA |
| CE certified | No, research use only |
| Brand | NucleoMag |
| Format | Magnetic beads |
| Handling | Magnetic separation |
| Automated use | Yes |
| Automation support | Compatible with most open robotic platforms. Please visit www.mn-net.com/automation or consult our technical support for further information. |
| Sample material | Whole blood collected in SARSTEDT S-Monovette® RNA Exact, Zymo DNA/RNA Shield Blood Tubes, Tempus RNA Blood Tubes, or EDTA / Citrate blood collection tubes |
| Sample amount | 9 mL of stabilized blood from Tempus™ Blood RNA Tube (corresponds to 3 mL whole blood), 260 µL EDTA / Citrate blood, 820 µL of stabilized blood from SARSTEDT S-Monovette® RNA Exact (corresponds to approx. 200 µL whole blood), 820 µL stabilized blood from Zymo Research DNA/RNA Shield™ Blood Collection Tube (corresponds to approx. 270 µL whole blood) |
| Fragment size | > 200 nt |
| Typical yield | 3-4 µg (strongly depends on sample quality and health status of donor) |
| Theoretical binding capacity | 0.4 µg/µL beads |
| Typical RIN | ≥ 6-10 (strongly depends on sample storage and quality) |
| Elution volume | 50–100 µL |
| Preparation time | Approx. 90 min for automated protocol, Approx. 120 min for manual protocol |
| Typical downstream application | cDNA library construction, Microarray spotting, Next Generation Sequencing, NGS, other enzymatic reactions, qPCR, RNA-Seq, RT−PCR |
| Storage temperature | 15–25 °C / 59–77 °F |
| Shelf life (from production) | 24 Month(s) |
| Hazardous material | Yes |
NucleoMag RNA Blood – Purification of RNA from whole blood
- Automated RNA purification from blood
- Minimal hands-on time and convenient handling
- Works with S-Monovette® RNA Exact, DNA/RNA Shield™, Tempus™ RNA Blood Tubes, and EDTA / Citrate blood
Highlights
- Maximum flexibility – works with multiple blood tube types
- High quality RNA for downstream applications
- Seamless integration into automated workflows
- Minimal hands-on: No pelleting- No reagent removal*
- Convenient handling: processing at room temperature*
Compatibility
- S-Monovette® RNA Exact (SARSTEDT)
- DNA/RNA Shield™ Blood Tubes (Zymo Research)
- Tempus™ RNA Blood Tubes (Thermo Fisher Scientific)*
- EDTA / Cirate blood tubes*
Specifications
| Technology | Magnetic beads |
| Processing | Manual or automated |
| Sample Type | Whole blood |
| Typcial yield | 3–4 µg |
| Elution volume | 50–100 mL |
| Purfification time | Approx. 90 min* |
Application data
Comparative analysis of RNA yield and purity from SARSTEDT S-Monovette® RNA Exact and Zymo DNA/RNA Shield™ Blood Tubes
Blood specimens sourced from two distinct donors were collected using SARSTEDT S-Monovette® RNA Exact or Zymo DNA/RNA Shield™ Blood Tubes, followed by purification employing the NucleoMag RNA Blood Kit via automated protocols on the MagnetaPure 32 Plus or KingFisher® Duo platform (n=3 per tube and instrument). Purified RNA underwent UV spectrometry analysis to assess total yield (blue bars) and purity (A260/280, orange dots). The data underscore remarkable reproducibility in RNA yield and purity across different donors, blood collection tubes, and automation platforms.
Robust RNA integrity profiles in S-Monovette® RNA Exact-collected samples
Blood specimens were procured from two distinct donors utilizing SARSTEDT S-Monovette® RNA Exact tubes and subjected to purification employing the NucleoMag RNA Blood kit via the automated MagnetaPure 32 Plus magnetic-rod extractor. Processing occurred subsequent to storage at room temperature for 2 hours (T0), 5 days (D5), or following two weeks of refrigeration at 2–8°C. Analysis conducted utilizing the Agilent Pico-Chip revealed excellent RNA integrity (8.0 ± 0.19) persisting across all examined time points and donors (n=3 for each donor and time point).
Comparative Analysis of RNA Yield and Purity from SARSTEDT S-Monovette® RNA Exact and Zymo DNA/RNA Blood Tubes
Blood specimens sourced from two distinct donors were collected using SARSTEDT S-Monovette® RNA Exact or Zymo DNA/RNA Blood Tubes, followed by purification employing the NucleoMag RNA Blood Kit via automated protocols on the MagnetaPure 32 Plus or KingFisher® Duo platform. Purified RNA underwent UV spectrometry analysis to assess total yield (blue bars) and purity (A260/280, orange dots). The data underscore remarkable reproducibility in RNA yield and purity across different donors, blood collection tubes, and automation platforms utilized.
Assessment of RNA qRT-PCR performance across multiple donors and automation platforms
Blood specimens obtained from three diverse donors were collected utilizing the SARSTEDT S-Monovette® RNA Exact Blood Tube, followed by purification employing the NucleoMag RNA Blood Kit through automated protocols on either the MagnetaPure 32 Plus (MP 32 Plus) or the KingFisher® Duo (KF Duo) platform. Subsequent to purification, RNA samples underwent qRT-PCR analysis employing a Taqman® Probe for a 73 bp Actin amplicon (blue squares) and a 357 bp FOS amplicon utilizing the SensiFast™ Probe Lo-ROX One-Step Kit from Bioline on an Applied Biosystems® 7500 Real-Time PCR System. The data reveal consistent RNA performance across multiple donors and automation platforms.
Comprehensive Evaluation of RNA Integrity and Performance from Tempus™ RNA Blood Tubes
Blood specimens were obtained from six distinct donors using Tempus™ RNA Blood Tubes and subjected to purification utilizing the NucleoMag RNA Blood kit via automated protocols on the KingFisher® DUO (left) or the MagnetaPure 32 Plus (right) magnetic-rod extractors. Subsequent analysis included UV spectrometry (A) for total yield (blue bars) and purity (A260/280, orange dots). Additionally, RNA integrity was assessed using UV spectrophotometry with the Agilent Pico-Chip, revealing exceptional integrity (7.7±0.5) across all samples and donors. Furthermore, purified RNA underwent qRT-PCR analysis (B) employing Taqman® Probes for 73 bp Actin and 357 bp FOS amplicons with the SensiFast™ Probe Lo-ROX One-Step Kit on an Applied Biosystems® 7500 Real-Time PCR System. The findings demonstrate consistent RNA quality and performance across diverse donors and automation platforms.
Blood tubes are not provided and must be purchased separately. The trade marks mentioned herin are the property of their respective owners. NucleoMag is a registered trademark of MACHEREY-NAGEL. Tempus™ is a registered trademark of Life Technologies. DNA/RNA Shield™ is a registered trademark of Zymo Research Corporation. Monovette® is a registered trademark of SARSTEDT. KingFisher® is a registered trademark of Thermo Scientific Inc.; Bioanalyzer® is a registered trademark of Agilent Technologies; SYBR® is a registered trademark of Life Technologies, TaqMan® is a registered trademark of Roche Molecular Systems, Inc