Description
NucleoMag HMW DNA can be used for the isolation of high molecular weight DNA from cells, human and animal tissue, plant material, whole blood, saliva, swabs, as well as bacteria and yeast samples.The magnetic bead technology streamlines your workflow and enhances reliability of HMW DNA isolation due to its compatibility with diverse automation platforms.
| Typical purity A260/A230 | ≥ 2.0, depending on sample type and quality |
| Typical recovery | Depending on sample amount and quality |
| Application | Isolation of DNA |
| Target | DNA |
| CE certified | No, research use only |
| Technology | Magnetic bead technology |
| Brand | NucleoMag |
| Format | Magnetic beads |
| Handling | Magnetic separation |
| Lysate clarification | Centrifugation |
| Automated use | Yes |
| Automation support | Compatible with most open robotic platforms. Please visit www.mn-net.com/automation or consult our technical support for further information. |
| Sample material | Animal tissue, Bacteria, Blood, Cells, Human tissue, Plant, Saliva, Swabs, Yeast |
| Sample amount | ≤ 20 mg human/animal tissue, ≤ 10⁶ cultured cells, ≤ 50 mg plant leaves, ≤ 25 mg gram-negative bacteria, ≤ 35 mg gram-positive bacteria, ≤ 50 mg yeast |
| Fragment size | ≥ 50 kb - Up to 100-200 kb |
| Typical purity A260/A280 | ≥ 1.8 , depending on sample type and quality |
| Elution volume | 100–200 µL |
| Preparation time | 60-120 min / 48 preps depending on manual or automated processing |
| Typical downstream application | Long-read sequencing, Next Generation Sequencing |
| Shelf life (from production) | 24 Month(s) |
| Gross weight (incl. packaging) | 807.6 g / 1.78 lbs |
| Packaging dimensions | 150 x 150 x 117 mm / 5.91 x 5.91 x 4.61 Inch |
| Hazardous material | Yes |
Safety Data Sheet: 744160.1_en-EN_GHS.pdf
- Download Instruction NucleoMag HMW DNA
- Download Flyer-NucleoMag-HMW-DNA-EN
- Download Leaflet NucleoMag HMW DNA
High molecular weight DNA from diverse sample materials
Genomic DNA from different samples such as heart tissue, plant leaves, blood, cells, saliva, bacteria and yeast was isolated using the NucleoMag HMW DNA Kit from MACHEREY-NAGEL. Isolated DNA shows fragment lengths above 50 kb. Samples were analyzed on Agilent’s Bioanalyzer 2100. Results obtained from different fragment length analyses.
High yields and purities
HMW DNA was extracted from different sample types with the NucleoMag HMW DNA and with Supplier Z kit. MACHEREY-NAGEL´s magnetic bead based kit showed higher yields as well as purities for the tested sample types. Isolated DNA was analyzed by NanoDropTM 2000.