Description
Anion exchange chromatography for nucleic acids of highest integrity
Specifications:
Application | Isolation of RNA |
Target | RNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Maxi prep |
Package unit | 10 Prep(s) |
Handling | Gravity flow |
Lysate clarification | Centrifugation |
Automated use | No |
Sample material | Animal tissue, Bacteria, Cells, Human tissue, Yeast |
Sample amount | 2 x 10⁷ |
Fragment size | 50 nt−approx. 300 knt |
Typical yield | 300 μg (2 x 10⁷ cultured cells), 150 µg (100 mg tissue), 200 µg (2 x 10⁹ bacteria) |
Theoretical binding capacity | 400 µg |
Typical purity A260/A280 | 1.8−1.95 |
Preparation time | 1.5–2.5 hour |
Typical downstream application | Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq |
Storage temperature | 15−25 °C |
Shelf life (from production) | 27 Months |
Scope of delivery | NucleoBond AXR 400 Columns, buffers, Urea |
Hazardous material | Yes |