Description
Anion exchange chromatography for nucleic acids of highest integrity
Specifications:
| Application | Isolation of RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Package unit | 25 Prep(s) |
| Handling | Gravity flow |
| Lysate clarification | Centrifugation |
| Automated use | No |
| Sample material | Animal tissue, Bacteria, Cells, Human tissue, Yeast |
| Sample amount | 5 x 10⁶ |
| Fragment size | 50 nt−approx. 300 knt |
| Typical yield | 70 μg (5 x 10⁶ cultured cells), 30 μg (20 mg tissue), 50 μg (5 x 10⁷ bacteria) |
| Theoretical binding capacity | 80 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Preparation time | 1.5–2.5 hour |
| Typical downstream application | Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 27 Months |
| Scope of delivery | NucleoBond AXR 80 Columns, buffers, Urea |
| Hazardous material | Yes |