Description
Anion exchange chromatography for purification of DNA from whole blood and cultured cells
Specifications:
| Application | Isolation of DNA |
| Target | DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Package unit | 20 Prep(s) |
| Handling | Gravity flow |
| Automated use | No |
| Sample material | Blood, Buffy coat, Cells |
| Sample amount | 2–5 mL whole blood or plasma, < 250 µL buffy coat, 2 x 10⁷ cultured cells |
| Fragment size | 500 bp–approx. 300 kbp |
| Typical yield | 100 µg |
| Theoretical binding capacity | 100 µg |
| Typical purity A260/A280 | 1.8–1.9 |
| Isopropanol precipitation | Centrifugation |
| Preparation time | 4–5 hour |
| Typical downstream application | PacBio sequencing |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Scope of delivery | NucleoBond AXG 100 Columns, Saccharose, buffers, Proteinase K |
| Hazardous material | Yes |