Description
Anion exchange chromatography for purification of high integrity DNA from different sample materials
Specifications:
| Shelf life | 39 Months |
| Application | Isolation of DNA |
| Target | DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Package unit | 2 Prep(s), 20 Prep(s) |
| Handling | Gravity flow |
| Automated use | No |
| Sample material | Animal tissue, Bacteria, Blood, Body fluids, Cells, Human tissue, Plant, Yeast |
| Sample amount | < 300 mg human/animal tissue, < 10⁷ cultured cells, < 1.5 g plant leaves, < 30 mg bacteria/yeast (ground under liquid nitrogen), < 300 mg bacteria/yeast (lysed enzymatically or by bead beating), < 2 mL liquid sample |
| Fragment size | 2 kbp–approx. 150 kbp (enzymatic lysis)/50 kbp (mechanical lysis) |
| Typical yield | Depending on amount and quality of sample |
| Theoretical binding capacity | 600 µg |
| Typical purity A260/A280 | 1.8–1.9 |
| Elution volume | 50–250 µL |
| Preparation time | 2 h/12 preps (incl. 30 min lysis) |
| Typical downstream application | Long-read sequencing, Next Generation Sequencing |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Hazardous material | Yes |