UST’s Transposase Enzyme-Linked Long-read Sequencing (UST TELL-Seq™) is a simple and scalable NGS library technology that generates barcode linked-reads for genome-scale sequencing applications. The whole procedure can be carried out in a PCR tube without the need for expensive instrumentation. The TELL-Seq™ Whole Genome Sequencing (WGS) Library Prep Kit will generate an Illumina sequencing library in just 3 hours. The protocol can be easily adjusted based on the genome size to be analyzed. TELL-Seq will be the new standard library method for WGS.
TELL-Seq™ Library Preparation and Sequencing Workflow
TELL-Seq Library Preparation and Sequencing Workflow
How it works
Results
RESULTS I: IGV SNAPSHOT OF TELL-SEQ LINKED READS
RESULTS II: TELL-SEQ RESULTS FOR THREE BACTERIA WITH DIFFERENT GC CONTENT
RESULTS II: TELL-SEQ RESULTS FOR THREE BACTERIA WITH DIFFERENT GC CONTENT
RESULTS III: DE NOVO ASSEMBLY RESULTS COMPARED WITH REFERENCE
RESULTS IV: WHOLE GENOME HAPLOTYPE PHASING RESULTS
Applications:
DE NOVO ASSEMBLY
Using the TELL-Seq™ WGS library prep kit you can sequence novel genomes. This works for small bacterial genomes, medium sized insect, plant, and fungi genomes and large animal and plant genomes. The TELL-Seq™ kit will turn an ordinary short-read 2nd generation DNA sequencer into a long-read 3rd generation DNA sequencer. If you really want to understand a new genome, including gene synteny, you must look at long-reads.
METAGENOMICS SEQUENCING
Samples containing disparate microbes are essential to gaining insight into our ecosystem as well as human disease. The TELL-Seq™ WGS Library Prep kit will allow you to sequence samples at a metagenomic scale; providing long-read like results with the ease of short-read sequencing. To see what is truly in a mixed microbial sample use the power of long-read DNA sequencing with the simplicity of short-read sequencing.
STRUCTURAL VARIATION
Short-read DNA sequencers have trouble identifying large structural variants. These could be in the form of copy number variants, translocations, duplications, deletions, insertions, and inversions. The TELL-Seq™ WGS Library Prep kit links short-reads turning them into long-reads. The linked, long-read data that you get using the TELL-Seq™ kit will allow you to take a step back and see what you were missing.
WHOLE GENOME PHASING
Knowing which homologous chromosome has which sequence is essential to understanding a genome. Whole Genome Phasing is difficult with 2nd generation, short-read, DNA sequencing technology. Phasing provides haplotype information which is important for understanding complex traits and variant linkages. The TELL-Seq™ WGS DNA Library Prep kit helps with this by linking short-reads to larger fragments and therefore long-reads.