Description
Say goodbye to complicated, time-consuming protein extraction workflows. The Covaris truPREP Protein 12x8 Strip Kit – Mammalian Cells (Lysis, Extraction, & Digestion) enables fast and reliable protein sample preparation from cell pellets in a single-consumable workflow, resulting in peptides for downstream LC-MS research. The comprehensive sample preparation process ensures consistency and reproducibility in a simple, streamlined workflow.
The kit accommodates a range of initial cell counts, from 1,000 up to 500,000 cells, offering flexibility and scalability in your experimental design. Developed with the Protein Aggregation Capture (PAC) workflow, this kit is designed for use with the Covaris R230 Focused-ultrasonicator, leveraging Covaris' Adaptive Focused Acoustics® (AFA®) technology to optimize efficiency and reduce turnaround time.
Workflow
Kit Contents
This kit includes enough of the following reagents and consumables to process 96 samples:
| Magnetic Bead Mixture | Reducing Reagent | 8 AFA-TUBE TPX Strip (12) |
| Wash Buffer Diluent | Alkayling Reagent | 8 AFA-TUBE TPX Strip Caps (12) |
| Tissue Lysis Buffer | Elution Buffer | Strip Prep Station (2) |
Note: Trypsin and accessory parts are not included in the kit and must be purchased separately. Accessories can be found below under Associated Products. For trypsin recommendations, please review the protocol.
| Technical Specification | Description |
|---|---|
| Part Number | 520354 |
| Sample Volume/ Sample Size | 1,000-500,000 Cells |
| Consumable | 8 AFA-TUBE TPX Strip (12) |
| Sample Type | Mammalian Cells |
| Application | Lysis, Extraction, Digestion |
| Instrument Compatibility | R230 |
| Sample Processing Per Kit | 96 |