truCOVER® Total RNA Library Prep Kits

• Sample Type: Total RNA samples from cells and tissues of human, mouse, rat (HMR) & related mammalian species. Optimized for FFPE samples.
• Application: Whole Transcriptome Sequencing, Stranded Total RNA Sequencing

Clinical and biopharmaceutical labs can't afford compromise. When samples are degraded, timelines are tight, and data quality is non-negotiable, your RNA library prep must perform - every single time, for every single sample.

Overcome the challenges with processing degraded RNA.

High-Performance RNA Library Prep for Challenging Samples

Covaris truCOVER^® Total RNA Library Prep is built to dominate the toughest and most complex RNA challenges. From pristine samples to severely degraded FFPE, truCOVER Total RNA Library Prep delivers robust, reliable, powerful, and strand-specific libraries with uncompromising precision, faster than ever before.

Driven by class-leading Adaptive Focused Acoustics^® (AFA^®) Technology, truCOVER Total RNA Library Prep maximizes RNA recovery, enforces precise and reproducible fragmentation, and significantly cuts down on workflow complexity, while reducing total working time, total number of steps, and overall sequencing costs.

This isn’t incremental improvement. It’s a workflow upgrade. If your lab demands confidence in gene fusion detection, low-input performance with solid reproducibility; this is your solution.

Key features of truCOVER Total RNA Library Prep Kits:

• Confident fusion detection: Detect gene fusions and alternative splicing events at lower sequencing depth, because real discoveries can’t wait.
• Consistent performance with challenging samples: Handle degraded FFPE with confidence; with DV200 values down to 30% without sacrificing quality. Optimize your FFPE sample's extraction-to-sequencing workflow by pairing with Covaris truXTRAC^® FFPE Kits.
• Maximum recovery, minimum waste: Extract powerful libraries from as little as 10 ng up to 500 ng of RNA input.
• Speed without sacrifice: Complete library prep in nearly 4 hours. Cut hands-on time by up to 40% compared to traditional methods. Automation-ready. Lab-efficient. Deadline-proof.
• Ultra-clean transcriptomes: rRNA depletion so efficient it drives cytoplasmic and mitochondrial rRNA reads below 1%, unlocking clearer biology and deeper insight.
• Precision that repeats: AFA-driven consistency ensures reproducible results across runs, operators, and sample types.
• Consistently higher proportion of exonic reads:  30-40% - Maximizes high-value data for differential gene expression (DGE).
• Better data, bigger ROI: Higher-quality data in less time means smarter sequencing spend and stronger downstream outcomes.

When degraded RNA tries to slow you down, it’s time to outperform it.

When workflows waste time, it’s time to change them.

When reproducibility matters, it’s time to turn expectation into routine performance.

truCOVER Total RNA Library Prep. Built for labs that refuse to compromise.

truCOVER Total RNA Library Prep Kits require Covaris Unique Dual Index (UDI) sets. 
Dual indexing enables the addition of adapters needed to bind to the flow cell for sequencing and minimizes index misassignment, particularly important for low-input, FFPE, or other degraded RNA samples.

Covaris UDI Plates for truCOVER Total RNA Library Prep can process up to 96 samples, either in a single run or during multiple successive runs. Covaris offers 8 UDI set options to support flexible multiplexing needs.

A complete AFA-powered workflow for RNA-Seq Library Prep

truCOVER Total RNA Library Prep Kit Contents

Covaris RNA UDI Set Content