Description
ExpressPlex 2.0: The workflow simplicity of one-step library prep combined with the performance of TnX for high throughput sequencing of plasmids, amplicons and microbial whole genomes.
- Improved performance: TnX transposase for reduced bias and improved coverage uniformity
- Ease of use: Fragmentation, barcoding, and amplification in a single step
- Rapid: 100-minute workflow (30 minutes or less hands-on) with pre-plated, reaction-ready reagents
- Flexible: 96- or 384-well plate format
- Scalable: High-level sample multiplexing (up to 6144) and auto normalization
ExpressPlex 2.0 Specifications
| ExpressPlex 2.0 Specifications | |
| Primary Applications | Plasmid & amplicon sequencing, and microbial whole genome sequencing (WGS) |
| Sample Input types | Purified plasmid DNA, RCA-amplified DNA, amplicons >350bp, colony PCR amplicons, microbial genomic DNA |
| Transposase | TnX – Next generation engineered transposase |
| Kit format |
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| DNA Input Recommended | 96-well format:
384-well format:
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| Total Library Prep Time | 100 minutes (30 minutes hands-on time) |
| Indexing Method | Combinatorial Dual Indexing |
| Number of Unique Index Combinations | Up to 6144 |
| Batch Size |
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| Output Fragment Range* | 400 – 1,200bp |
| Number of PCR Cycles |
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| Sequencer Compatibility |
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* Fragment size will depend on magnetic bead cleanup ratios used.
ExpressPlex 2.0 Library Prep Kit Includes:
- Indexing Reaction Plate(s)
- Ready Reaction Mix Plate(s)
- MAGwise Paramagnetic Beads
- Users do not need to supply polymerase, primers, or barcodes. These are contained within the supplied ready reaction mix.
ExpressPlex 2.0 is an automation-friendly, one-step library prep powered by TnX to reduce bias and improve coverage uniformity using a highly streamlined workflow for sequencing synthetic constructs like plasmids & amplicons as well as microbial WGS.
TnX Transposase: Quality Libraries for Quality Sequencing
ExpressPlex 2.0 uses tagmentation powered by TnX, the next generation transposase, to simultaneously fragment & index sample DNA. Average fragment size ranges from 400-1200 bp.
Typical fragment profile following analysis on an Agilent TapeStation System. Data shown are for a 96-plex sample.
TnX: Transposase Engineered for Reduced Insertion Bias
Better transposase = better sequencing. Take your plasmid and amplicon screening to the next level!
ExpressPlex 2.0 is the only library prep kit powered by TnX! TnX was engineered to reduce insertional bias to improve uniformity of coverage. When directly compared to a Tn5-based competitor library prep kit for sequencing of 2 different plasmids, ExpressPlex 2.0 produced significantly lower CVs and range of coverage (max/min).
Library preparation for pUC19 and pMal-c67 was conducted using ExpressPlex 2.0 kit and a Tn5-based competitor kit using standard manufacturers’ protocols. Libraries were sequenced using an Illumina MiSeq.
Product Highlights
Auto-Normalization of Read Depth
ExpressPlex demonstrates significantly higher levels of normalization compared to competitors, enabling a simplified workflow where individual normalization is no longer required to achieve more consistent read-depths across samples.
Libraries were prepared from three different reference plasmids of varying sizes across a 10-fold input range at full reaction volume for ExpressPlex (16 µL) and Nextera XT (50 µL), as well as at ¼ miniaturized reaction volume for Nextera XT.
For each method, inputs ranged from 4-40ng for ExpressPlex (standard input is 16ng) and 0.4 – 4ng for Nextera XT (standard input is 1ng). Inputs were scaled appropriately for 1/4 reactions.
Auto-Normalization of Insert Size
ExpressPlex generates highly consistent library insert sizes over a broad range of DNA inputs.
Libraries from pUC19 plasmid DNA were simultaneously prepared at 3 different input amounts (n=8 each input amount). ExpressPlex demonstrated greater consistency in average insert size across a 10-fold range. In each case, the standard input for both kits (16ng for ExpressPlex, 1ng for Nextera XT) was also included as a mid-range data point.
ExpressPlex 2.0: 96-well workflow
ExpressPlex 2.0: 384-well workflow
RECORDED WEBINAR
Moderna presentation – Antibody Sequencing: New Operating Model
In this presentation, Nasthas Lacerda Almeida from Moderna, highlights how her organization has leveraged ExpressPlex™ 2.0 for a clonal antibody sequencing approach, optimized for speed and scalability using de novo assembly of NGS short reads.
Downloads:
ExpressPlex 2.0 (96- & 384-well) Library Preparation Kit Automation Guide
ExpressPlex 2.0 Technical Specification Sheet
ExpressPlex 2.0 User Guide: 96-well format