Short Read NGS Library Construction – For Inserts from 100bp to 1.5kb
The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross-contamination.
Benefits of the Pippin Prep System:
- Saves time and effort compared to manual gel purification
- Sample-to-sample reproducibility streamlines research
- Higher sample quality improves downstream analysis
Key Downstream Technologies:
- Paired-end sequencing
DNA Gel Cassettes
Pre-Cast Agarose Gel Cassettes for DNA Size Selection
Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100bp and 1.5kb. For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples per cassette). Run times are as little as 30 minutes. View the Pippin Prep cassette reference chart to explore the DNA size selection options and run times.
Gel cassette types and target ranges
- 3% agarose, 100-250bp
- 2% agarose, 100-600bp
- 1.5% agarose, 250bp – 1.5kb
See the Workflow Summary:
For these downstream technologies
- miRNA isolation
- DNA sequencing, RNA-seq, genotyping
- DNA sequencing, ChIP-seq
- Mate-pair sequencing
The Pippin Prep instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.
|Pippin Prep Specs
||100V or 150V constant
||535 nm excitation | 640 nm emission
||100-240 VAC, 2.5 A, 50-60 Hz
||15 lbs / 7 kg
||7h x 11w x 21d (in) | 18h x 28w x 53 d (cm)
Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.