DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field
(100bp – 50kb)
Size-Select DNA Fragments up to 50kb Collect HMW DNA with High-Pass Filtering
The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user.
Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross contamination.
Benefits of the BluePippin System
- Saves time and effort compared to manual gel purification
- Sample-to-sample reproducibility streamlines research
- Higher sample quality improves downstream analysis
Key Downstream Technologies
- Mate-pair sequencing
- Single molecule sequencing
- Bar-coded linked reads
The BluePippin instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.
|Electrophoresis voltage||25V, 100V or 150V constant, 100V pulsed field|
|Optical detection||470 nm excitation, 525 nm emission|
|Power requirements||100-240 VAC, 2.5 A, 50-60 Hz|
|Weight||15 lbs / 7 kg|
|Dimensions||7h x 11w x 21d (in) | 18h x 28w x 53 d (cm)|
Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.