RevvitySKU: REV-NOVA-5149-23

Size: 96rxn


NEXTFLEX® Rapid XP V2 DNA-Seq Kit for Illumina® Platforms

  • Fully automated normalization & fragmentation
  • Enzymatic fragmentation with end-repair and A-tailing in one reaction
  • Highly efficient ligation with low adapter dimer formation
  • Efficient: Automated on the Sciclone® and Zephyr® NGS workstations for mid-high throughput automation and now on the BioQule™ NGS System for low throughput labs
  • Up to 1,536 UDI Barcodes available for multiplexing

The new NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples.


  • NEXTFLEX® Fragmentation Buffer V2
  • NEXTFLEX® Fragmentation Enzyme V2
  • NEXTFLEX® Ligation Master Mix V2
  • NEXTFLEX® PCR Master Mix V2
  • NEXTFLEX® Primer Mix V2
  • Nuclease-free Water
  • Elution Buffer
  • NEXTFLEX® Cleanup Beads XP
  • NEXTFLEX® Normalization Beads V2


  • 100 pg – 1 µg of DNA in up to 17.5 µL nuclease-free water.
  • NEXTFLEX® barcodes
  • Ethanol 100% (room temperature)
  • 96 well PCR Plate Non-skirted (Phenix® Research, Cat # MPS-499) or similar
  • 96 well Library Storage and Pooling Plate (Thermo Fisher® Scientific, Cat # AB-0765) or similar
  • Adhesive PCR Plate Seal (Bio-Rad®, Cat # MSB1001)
  • Magnetic Stand -96 (Thermo Fisher® Scientific, Cat # AM10027) or similar
  • Thermocycler
  • 2, 10, 20, 200 and 1000 µL pipettes / multichannel pipettes
  • Nuclease-free barrier pipette tips
  • Vortex

Highly Efficient Ligation with Low Adapter Formation

The NEXTFLEX® Rapid XP V2 DNA-seq kit offers a streamlined workflow with highly efficient ligation, generating low amount of adapter dimers even at low input concentrations.

Figure 1. Low dimer formation with NEXTFLEX® Rapid XP V2 DNA-Seq kit. Different library replicates were prepared using either 500 pg (A) or 1 ng (B) of high-quality genomic DNA as input. Adapter dimer should appear as a peak at ~150 bp.

Figure 2. Analysis of GC bias from libraries obtained with NEXTFLEX Rapid XP V2 DNA-Seq kit. Results show minimal variability in normalized coverage across 20-60% GC content encompassing ~95% of the human genome (Right: red bars). These are consistent across 1-750ng of genomic DNA input.

Simplify Library Construction with NEXTFLEX Normalization Beads

Flexible Multiplexing Options with 1,536 Barcodes Available for High-Throughput Multiplexing

Barcodes are not supplied with the NEXTFLEX® Rapid XP DNA-seq kit v2 and need to be purchased separately. We offer 384 color-balanced NEXTFLEX® UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs. Additionally, a set of 1,536 NEXTFLEX® UDI adapters which are compatible with the NEXTFLEX® Rapid XP DNA-seq kit v2 are now available for ultra high-throughput applications.

For more information about barcode options compatible with the NEXTFLEX® Rapid XP DNA-seq kit v2 and bundled pricing, please contact

Automation Compatibility

The NEXTFLEX® Rapid XP V2 DNA-Seq kit is compatible with multiple liquid handlers, including the Sciclone® G3 NGSx workstation, Sciclone® G3 NGSx iQ workstation, and Zephyr® NGS workstation.

For low throughput automation, the NEXTFLEX® Rapid XP V2 DNA-Seq kit is also now automated on the BioQule™ NGS System which simplifies both library prep AND quantitation. This low-cost, benchtop instrument delivers libraries ready to load into your sequencer with only 15 minutes of hands-on time. The kit developed for BioQule™ NGS System does not include normalization beads but the instrument quantifies each library before completion of the workflow.

For larger volume requirements, customization and bulk packaging is available. For increased flexibility, individual reagents (non-master mixed) are also available. Please contact for further information.


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