Description
The NEXTFLEX® Small RNA-Seq Kit v4 enables labs to reduce bias in their Illumina® and Element Biosciences® small RNA sequencing workflows while leveraging a completely gel-free protocol, even with low input samples.
For research use only. Not for use in diagnostic procedures.
Overview
The NEXTFLEX small RNA sequencing kit v4 uses patent-pending technology to provide a completely gel-free small RNA library preparation solution for Illumina sequencing platforms.
- Completely gel-free protocol from purified miRNA or 1 ng of total RNA
- Automated on the Sciclone® G3 NGSx, Sciclone G3 NGSx iQ™, and the Zephyr® G3 NGS workstations
- Patent pending technology optimized for challenging samples such as plasma or serum, including exosomes
- Exceptional miRNA discovery
- Ready to sequence libraries in ~6 hours
- Multiplexing of up to 384 samples
- An automation protocol is available
- Functionally certified with Illumina sequencing platforms
Additional product information
Gel-free small RNA library prep with reduced bias
The NEXTFLEX small RNA sequencing kit v4 uses patent-pending technology to provide a completely gel-free small RNA library preparation solution for Illumina sequencing platforms. Our approach to reducing bias involves optimization of the ligation reaction and the use of dimer reduction oligonucleotides. These changes result in decreased bias in comparison to standard protocols.
NEXTFLEX Small RNA-Seq Kit v4 allows preparation of libraries ready for sequencing with a simplified workflow in approximately 6 hours, with as little input as 1 ng of total RNA. Libraries prepared with this kit have a higher proportion of mapping reads and unique miRNAs than leading competitors (Figure 1 and 2).
Figure 1
Figure 2: Gel-free miRNA library prep from exosomes
This convenient gel-free workflow incorporated in the NEXTFLEX Small RNA-Seq Kit v4 enables the construction of libraries from exoRNA. Using this workflow, researchers can achieve a high number of reads aligning to mature miRNA and low adapter dimers, even with the very low miRNA inputs characteristic of exosome samples.
Figure 3: Average top 10 miRNA observed with exosomes from bone marrow and pooled serum samples.
Maximize miRNA mapped reads with NEXTFLEX blood miRNA blockers
The NEXTFLEX blood miRNA blockers deplete common miRNA in blood and plasma such as miR-486-5p, miR-92a-3p and miR-451a. These constitute 50-70% of the small RNA present in those samples and usually are not of interest for researchers.
Small RNA sequencing library prep optimized for automation
By eliminating all gel-based steps even for low input samples, the NEXTFLEX Small RNA-Seq Kit V4 is optimized for easy migration onto automated liquid handling platforms. This workflow is automated on the Sciclone G3 NGSx, Sciclone G3 NGSx iQ, and the Zephyr G3 NGS workstations.
Illumina small RNA sequencing multiplexing with UDI barcodes
Up to 384 UDI barcoded primers are included in each kit for multiplexing. These UDIs allow libraries to be run on any Illumina sequencing platform. These include the Illumina NextSeq® and Novaseq® sequencers and only require standard Illumina sequencing primers.
Maximize miRNA mapped reads with NEXTFLEX blood miRNA blockers
The NEXTFLEX blood miRNA blockers deplete common miRNA in blood and plasma such as miR-486-5p, miR-92a-3p and miR-451a. These constitute 50-70% of the small RNA present in those samples and usually are not of interest for researchers.