Description
The DepleteX® Mitochondrial DNA Depletion kit employs a straightforward CRISPR-based method to selectively remove unwanted human molecules derived from mitochondrial nucleic acid before sequencing. Researchers can achieve more informative results while sequencing less in samples with abundant mitochondrial content such as ATAC-seq NGS libraries.
For research use only. Not for use in diagnostic procedures.
Overview
Efficiently remove mitochondrial reads from DNA or RNA samples
Mitochondrial reads consume significant sequencing space in certain sample type and applications. Depleting these reads prior to sequencing allows redirection of sequencing resources toward valuable information for researchers.
The DepleteX® Mitochondrial DNA Depletion kit enhances sequencing by eliminating mitochondrial reads from DNA or RNA NGS libraries before sequencing.
The DepleteX Mitochondrial DNA Depletion kit is used after DNA or RNA library preps and prior to sequencing. This kit is compatible with most DNA-seq & RNA-seq workflows; however, we recommend using it with the NEXTFLEX™ Rapid XP V2 DNA-seq kit or NEXTFLEX™ RNA-seq 2.0 Kit.
Figure 1 illustrates the majority of mitochondrial reads can be eliminated from human samples. This depletion allows for the enrichment of rare and less abundant sequences in NGS libraries.
Additional product information
Figure 1. The DepleteX ® Mitochondrial DNA Depletion kit removed the majority of mitochondrial reads from a human tissue sample.
Specifications
| Automation Compatible |
No
|
|---|---|
| Product Group |
Ribodepletion
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Unit Size |
24 rxns
|