The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTfFLEX® Unique Dual Index Barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
The NEXTFLEX® Unique Dual Index Barcodes contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex® Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.