Protino Ni-NTA Columns 5 mL

Macherey-NagelSKU: MN-745415.1

Size: 1


Method of choice for His-tag protein purification with best performance in ready to use 5 mL FPLC columns



Application Purification of His-tag proteins
Target Protein
CE certified No, research use only
Technology Immobilized metal ion affinity chromatography
Chelating ligand NTA (nitrilotriacetic acid)
Brand Protino
Format FPLC columns
Package unit 1 Piece(s), 5 Piece(s)
Handling MPLC, FPLC, peristaltic pump, or syringe
Automated use No
Sample material Protein solution
Matrix 6 % beaded agarose (cross-linked), precharged with Ni²⁺
Bead size 45–165 µm
Theoretical binding capacity 250 mg
Recommended linear flow rate 5 mL/min
Max linear flow rate 10 mL/min
Storage temperature 4−8 °C
Shelf life (from production) 24 Months
Hazardous material Yes




Purification Guide

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