NucleoSpin totalRNA FFPE

Macherey-NagelSKU: MN-740982.10

Size: 10


Mini spin kit for isolation of small and large RNA from formalin-fixed, paraffin-embedded samples



Application Isolation of total RNA
Target RNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Package unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification Centrifugation
Automated use No
Sample material FFPE, Fixed samples, Tissue sections
Sample amount 10 sections (10 μm) with < 50 mg of tissue
Fragment size > 18 nt
Typical yield Depending on amount and quality of sample
Theoretical binding capacity 200 µg
Elution volume 30–50 µL
Preparation time 70 min/6 preps (90 min incl. optional rDNase digest)
Typical downstream application Array technology, Northern blotting, Primer extension, qRT−PCR, RNase protection assays
Storage temperature 15−25 °C
Shelf life (from production) 24 Months
Hazardous material Yes




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