Description
Midi spin kit for isolation of RNA of highest integrity
Specifications:
| Application | Isolation of RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Midi prep |
| Package unit | 20 Prep(s) |
| Handling | Centrifugation |
| Lysate clarification | Centrifugation, NucleoSpin Filter Midi |
| Automated use | No |
| Sample material | Animal tissue, Bacteria, Cells, Human tissue, RNA solution, Yeast |
| Sample amount | < 5 x 10⁷ cultured cells, < 200 mg human / animal tissue , < 10¹⁰ bacterial cells, < 3 x 10⁸ yeast cells |
| Fragment size | > 200 nt |
| Typical yield | 180 µg (10⁷ HeLa cells), 620 µg (4 x 10⁷ HeLa cells) |
| Theoretical binding capacity | 700 µg |
| Typical purity A260/A280 | 1.9–2.1 |
| Typical RIN | > 9 |
| Elution volume | 500–1000 µL |
| Preparation time | 80 min/4 preps |
| Typical downstream application | Chip hybridization, Northern blotting, qRT−PCR, RNA-Seq |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Scope of delivery | NucleoSpin RNA Midi Columns, NucleoSpin RNA Filters Midi, Elution Tubes, buffers, RNase-free rDNase |
| Hazardous material | Yes |