NucleoSpin RNA

Macherey-NagelSKU: MN-740955.250

Size: 250


Mini spin kit for isolation of RNA of highest integrity



Application Isolation of RNA
Target RNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Package unit 10 Prep(s), 50 Prep(s), 250 Prep(s), QIAcube 240 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification Centrifugation, NucleoSpin Filter
Automated use No
Sample material Animal tissue, Bacteria, Cells, Human tissue, Yeast
Sample amount < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 10⁹ bacterial cells, < 10⁸ yeast cells
Fragment size > 200 nt
Typical yield 14 µg (10⁶ HeLa cells), 70 µg (10⁹ bacterial cells)
Theoretical binding capacity 200 µg
Typical purity A260/A280 1.9–2.1
Typical RIN > 9
Elution volume 30–120 µL
Preparation time 35 min/6 preps
Typical downstream application RNA-Seq, RT−PCR
Storage temperature 15−25 °C
Shelf life (from production) 24 Months
Hazardous material Yes




Support Protocols:


User Guide - RNA purification guide

Application Note - RNA isolation from bacteria and yeast

Tech Note - RNA isolation from insects

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