Description
Larce scale purification of high quality DNA from blood
Specifications:
| Application | Isolation of DNA |
| Target | DNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Maxi prep |
| Package unit | 10 Prep(s), 50 Prep(s) |
| Handling | Centrifugation |
| Automated use | No |
| Sample material | Blood, Body fluids, Buffy coat, Cells, Plasma, Platelets, Serum |
| Sample amount | 2–10 mL whole blood (human or animal, fresh or frozen, treated with citrate, EDTA, heparin, or CPDA), buffy coat, platelets, body fluids, serum, plasma, 10⁸ cultured cells |
| Fragment size | 200 bp–approx. 50 kbp |
| Typical yield | 200–300 µg |
| Typical concentration | 200–500 ng/µL |
| Theoretical binding capacity | 700 µg |
| Typical purity A260/A280 | 1.6–1.9 |
| Elution volume | 600-2000 µL |
| Preparation time | 60 min/prep |
| Typical downstream application | enzymatic reactions, Next Generation Sequencing, PCR, Southern blotting |
| Storage temperature | 15−25 °C (Columns to be stored at 4 °C upon arrival) |
| Shelf life (from production) | 19 Months |
| Hazardous material | Yes |