NucleoSpin RNA/Protein

Macherey-NagelSKU: MN-740933.10

Size: 10


Parallel isolation of RNA and protein from an undivided sample



Application Isolation of RNA and protein
Target RNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Package unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Handling Centrifugation
Lysate clarification Centrifugation, NucleoSpin Filter
Automated use No
Sample material Animal tissue, Cells, Human tissue, Plant
Sample amount < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 100 mg plant tissue
Fragment size RNA > 200 nt, protein 15–300 kDa
Typical yield < 70 µg RNA, < 1200 µg Protein
Theoretical binding capacity 200 µg
Typical purity A260/A280 1.9–2.1
Typical RIN > 9
Elution volume RNA 40–120 µL, protein 10–100 µL
Preparation time 30 min/6 preps (RNA), + 35 min/6 preps (protein)
Typical downstream application Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays
Storage temperature 15−25 °C
Shelf life (from production) 24 Months
Hazardous material Yes




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