Mini spin kit for isolation of total DNA from diverse soil types, MN Bead Tubes Type A included
|Application||Isolation of DNA|
|CE certified||No, research use only|
|Technology||Silica membrane technology|
|Package unit||10 Prep(s), 50 Prep(s), 250 Prep(s)|
|Lysate clarification||Centrifugation, NucleoSpin Inhibitor Removal Column|
|Sample material||Sediment, Sludge, Soil|
|Sample amount||< 500 mg|
|Fragment size||50 bp–approx. 50 kbp|
|Typical yield||2–10 µg (500 mg soil)|
|Theoretical binding capacity||50 µg|
|Elution volume||30–100 µL|
|Preparation time||90 min/10 preps|
|Typical downstream application||Microarrays, PCR, Real-time PCR, Southern blotting|
|Storage temperature||15−25 °C|
|Shelf life (from production)||27 Months|
Learn MoreNucleoSpin Soil
Soil samples contain different amounts of organic matter and inorganic material (humic substances or polysaccharides) and exhibit different phs, all of which can hinder the consistency of DNA extraction from these sample types. The NucleoSpin Soil kit overcomes these obstacles though the use of highly optimized lysis conditions and mechanical disruption.
NucleoSpin Soil procedure
Superior DNA yield and purity from various soil types
Two different lysis buffer options plus optional addition of the chemical additive Enhancer SX allow fine tuning for maximum yield and purity.
Complete removal of PCR inhibitors – PCR results even from undiluted eluates!
DNA was purified with NucleoSpin Soil using Lysis Buffer SL1 and Lysis Buffer SL2 with, and without. Enhancer SX as well as with kits from competitor MP, and MO. Then, 2 μL of undiluted eluate were used as PCR template with fungi specific internal transcribed spacer (ITS) primers. Competitor MP failed to yield DNA pure enough to be used undiluted. DNA, and inhibitor concentration were both low for competitor MO, but the PCR from river sediment samples was still strongly inhibited. With NucleoSpin Soil there were at least two conditions for each soil type yielding plenty of DNA, and working undiluted in PCR.
Efficient lysis system – even suitable for difficult-to-lyse microorganisms
Total DNA from 400 mg cropping soil was purified with NucleoSpin Soil using Lysis Buffer SL2 in combination with Enhancer SX. 2 μl of undiluted eluates were analyzed in PCR using, order specific primer systems.
Adamczyk et al. Plant roots increase both decomposition, and stable, organic matter formation in boreal forest soil. Nature Communications, volume 10, 3982 (2019).
Goncalves et al. Microbiota stimulation generates LCMV-specific memory CD8+ T cells in SPF mice, and determines their TCR repertoire during LCMV infection. Molecular Immunology, volume 124, pages 125–141 (2020).