NucleoSpin Plant II Midi

Macherey-NagelSKU: MN-740771.20

Size: 20


Medium scale isolation of DNA from a multitude of plant samples



Application Isolation of DNA
Target DNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Midi prep
Package unit 20 Prep(s)
Handling Centrifugation
Lysate clarification Centrifugation, NucleoSpin Filters Midi
Automated use No
Sample material Plant
Sample amount < 400 mg wet weight, < 80 mg dry weight
Fragment size 50 bp–approx. 50 kbp
Typical yield 10–100 µg (400 mg wet weight)
Theoretical binding capacity 200 µg
Typical purity A260/A280 1.8–1.9
Elution volume 200–400 µL
Preparation time 90 min/prep
Typical downstream application enzymatic reactions, PCR, Southern blotting
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Scope of delivery NucleoSpin Plant II Midi Columns, NucleoSpin Filters L, Collection Tubes, buffers, RNase A
Hazardous material Yes





Application Note

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