Description
Medium scale isolation of DNA from a multitude of plant samples
Specifications:
| Application | Isolation of DNA |
| Target | DNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Midi prep |
| Package unit | 20 Prep(s) |
| Handling | Centrifugation |
| Lysate clarification | Centrifugation, NucleoSpin Filters Midi |
| Automated use | No |
| Sample material | Plant |
| Sample amount | < 400 mg wet weight, < 80 mg dry weight |
| Fragment size | 50 bp–approx. 50 kbp |
| Typical yield | 10–100 µg (400 mg wet weight) |
| Theoretical binding capacity | 200 µg |
| Typical purity A260/A280 | 1.8–1.9 |
| Elution volume | 200–400 µL |
| Preparation time | 90 min/prep |
| Typical downstream application | enzymatic reactions, PCR, Southern blotting |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 27 Months |
| Scope of delivery | NucleoSpin Plant II Midi Columns, NucleoSpin Filters L, Collection Tubes, buffers, RNase A |
| Hazardous material | Yes |