NucleoBond RNA/DNA 400 (10)

Macherey-NagelSKU: MN-740651



Anion exchange chromatography for nucleic acids of highest integrity



Application Isolation of RNA
Target RNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Maxi prep
Package unit 10 Prep(s)
Handling Gravity flow
Lysate clarification Centrifugation
Automated use No
Sample material Animal tissue, Bacteria, Cells, Human tissue, Yeast
Sample amount 2 x 10⁷
Fragment size 50 nt−approx. 300 knt
Typical yield 300 μg (2 x 10⁷ cultured cells), 150 µg (100 mg tissue), 200 µg (2 x 10⁹ bacteria)
Theoretical binding capacity 400 µg
Typical purity A260/A280 1.8−1.95
Preparation time 1.5–2.5 hour
Typical downstream application Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Scope of delivery NucleoBond AXR 400 Columns, buffers, Urea
Hazardous material Yes





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