NucleoBond RNA/DNA 80 (25)

Macherey-NagelSKU: MN-740650



Anion exchange chromatography for nucleic acids of highest integrity



Application Isolation of RNA
Target RNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Package unit 25 Prep(s)
Handling Gravity flow
Lysate clarification Centrifugation
Automated use No
Sample material Animal tissue, Bacteria, Cells, Human tissue, Yeast
Sample amount 5 x 10⁶
Fragment size 50 nt−approx. 300 knt
Typical yield 70 μg (5 x 10⁶ cultured cells), 30 μg (20 mg tissue), 50 μg (5 x 10⁷ bacteria)
Theoretical binding capacity 80 µg
Typical purity A260/A280 1.8−1.95
Preparation time 1.5–2.5 hour
Typical downstream application Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Scope of delivery NucleoBond AXR 80 Columns, buffers, Urea
Hazardous material Yes






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