Description
Preparative scale isolation of endotoxin-free plasmid DNA
Specifications:
| Application | High and low copy plasmid DNA purification |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Preparative scale |
| Package unit | 1 Prep(s) |
| Handling | HPLC, FPLC |
| Lysate clarification | Folded filter, sieving fabric |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | 5−20 L |
| Vector size | < 300 kbp |
| Typical yield | 80−100 mg |
| Theoretical binding capacity | 100 mg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | < 0.1 EU/µg DNA |
| Isopropanol precipitation | Centrifugation |
| Preparation time | 20 h/prep |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Scope of delivery | NucleoBond AX Prep 100 Column, NucleoBond AX 100 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A |
| Hazardous material | Yes |