NucleoBond PC Prep 100 (1)

Macherey-NagelSKU: MN-740594



Preparative scale isolation of endotoxin-free plasmid DNA



Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Preparative scale
Package unit 1 Prep(s)
Handling HPLC, FPLC
Lysate clarification Folded filter, sieving fabric
Automated use No
Sample material Bacteria, E. coli
Sample amount 5−20 L
Vector size < 300 kbp
Typical yield 80−100 mg
Theoretical binding capacity 100 mg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation
Preparation time 20 h/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Scope of delivery NucleoBond AX Prep 100 Column, NucleoBond AX 100 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Hazardous material Yes




Additional Notes

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