NucleoBond PC 10000 (5)

Macherey-NagelSKU: MN-740593



1st generation anion exchanger for purification of plasmid DNA



Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Giga prep
Package unit 5 Prep(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 500−2000 mL (high copy), 1−4 L mL (low copy)
Vector size < 300 kbp
Typical yield 2000−10000 µg
Theoretical binding capacity 10000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 120−150 min/2 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Scope of delivery NucleoBond AX 10000 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Hazardous material Yes




Supplementary Note

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