NucleoBond BAC 100 (10)

Macherey-NagelSKU: MN-740579



1st generation anion exchange technology for large construct plasmid DNA



Application Large contruct (P1, BACs, PACs) plasmid purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format BAC prep
Package unit 10 Prep(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 100−500 mL E. coli culture
Vector size < 300 kbp
Typical yield 10−100 µg
Theoretical binding capacity 100 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation
Preparation time 120 min/2−4 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Scope of delivery NucleoBond BAC 100 Columns, NucleoBond Folded Filters, buffers, RNase A
Hazardous material Yes




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