NucleoBond PC 500

Macherey-NagelSKU: MN-740574

Size: (10)
Price:
$287

Description

1st generation anion exchanger for purification of plasmid DNA

 

Specifications:

Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Maxi prep
Package unit 10 Prep(s), 25 Prep(s), 50 Prep(s), 100 Prep(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 30−150 mL (high copy), 100−500 mL (low copy)
Vector size < 300 kbp
Typical yield 400−500 µg
Theoretical binding capacity 500 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 80−90 min/4 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transformation
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes

 

SDS

Manual

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