NucleoBond PC 500 EF (10)

Macherey-NagelSKU: MN-740550



1st generation anion exchange technology for endotoxin-free plasmid DNA



Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Maxi prep
Package unit 10 Prep(s)
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 30−150 mL
Vector size < 300 kbp
Typical yield 100−500 µg
Theoretical binding capacity 500 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 100 min/2 preps
Typical downstream application Automated or manual sequencing, Cloning, hybridization, In-vitro transcription, PCR, Transfection
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Scope of delivery NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A
Hazardous material Yes




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