NucleoBond PC 2000 EF (5)

Macherey-NagelSKU: MN-740549



1st generation anion exchange technology for endotoxin-free plasmid DNA



Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Mega prep
Package unit 5 Prep(s)
Handling Gravity flow
Lysate clarification Bottle top filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 150−500 mL
Vector size < 300 kbp
Typical yield 500−2000 µg
Theoretical binding capacity 2000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer Large, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 150 min/2 preps
Typical downstream application Automated or manual sequencing, Cloning, hybridization, In-vitro transcription, PCR, Transfection
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Scope of delivery NucleoBond AX 2000 Columns, NucleoBond Bottle Top Filters Type 1, buffers, RNase A
Hazardous material Yes




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