NucleoBond PC 10000 EF (5)

Macherey-NagelSKU: MN-740548



1st generation anion exchange technology for endotoxin-free plasmid DNA


Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Giga prep
Package unit 5 Prep(s)
Handling Gravity flow
Lysate clarification Bottle top filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 500−2000 mL
Vector size < 300 kbp
Typical yield 2000−10000 µg
Theoretical binding capacity 10000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation
Preparation time 180 min/2 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Scope of delivery NucleoBond AX 10000 Columns, NucleoBond Bottle Top Filters Type 2, buffers, RNase A
Hazardous material Yes





NucleoBond PC EF - For purification of endotoxin-free plasmid DNA

A particularly challenging aspect of such procedures is the presence of endotoxins - Endotoxins are residual components of bacterial cell walls that can trigger an inflammatory response and dramatically decrease the efficiency of transfection in addition to distorting any results obtained from experiments.

Endotoxin-free plasmid is a crucial requirement for transfections and gene therapy applications. For this reason, MACHEREY-NAGEL created a proprietary technique for reducing endotoxins to extremely low levels. Learn more about our NucleoBond PC 10000 kits in this workflow video: Link

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