Description
1st generation anion exchange technology for endotoxin-free plasmid DNA
Specifications:
| Application | High and low copy plasmid DNA purification |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Giga prep |
| Package unit | 5 Prep(s) |
| Handling | Gravity flow |
| Lysate clarification | Bottle top filter |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | 500−2000 mL |
| Vector size | < 300 kbp |
| Typical yield | 2000−10000 µg |
| Theoretical binding capacity | 10000 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | < 0.1 EU/µg DNA |
| Isopropanol precipitation | Centrifugation |
| Preparation time | 180 min/2 preps |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 27 Months |
| Scope of delivery | NucleoBond AX 10000 Columns, NucleoBond Bottle Top Filters Type 2, buffers, RNase A |
| Hazardous material | Yes |
NucleoBond PC EF - For purification of endotoxin-free plasmid DNA
A particularly challenging aspect of such procedures is the presence of endotoxins - Endotoxins are residual components of bacterial cell walls that can trigger an inflammatory response and dramatically decrease the efficiency of transfection in addition to distorting any results obtained from experiments.
Endotoxin-free plasmid is a crucial requirement for transfections and gene therapy applications. For this reason, MACHEREY-NAGEL created a proprietary technique for reducing endotoxins to extremely low levels. Learn more about our NucleoBond PC 10000 kits in this workflow video: Link