NucleoBond CB 100 (20)

Macherey-NagelSKU: MN-740508



Anion exchange chromatography for purification of DNA from whole blood and cultured cells



Application Isolation of DNA
Target DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Package unit 20 Prep(s)
Handling Gravity flow
Automated use No
Sample material Blood, Buffy coat, Cells
Sample amount 2–5 mL whole blood or plasma, < 250 µL buffy coat, 2 x 10⁷ cultured cells
Fragment size 500 bp–approx. 300 kbp
Typical yield 100 µg
Theoretical binding capacity 100 µg
Typical purity A260/A280 1.8–1.9
Isopropanol precipitation Centrifugation
Preparation time 4–5 hour
Typical downstream application PacBio sequencing
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Scope of delivery NucleoBond AXG 100 Columns, Saccharose, buffers, Proteinase K
Hazardous material





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