NucleoSpin Plasmid (NoLid)

Macherey-NagelSKU: MN-740499.10

Size: 10


Rapid Mini preparation of plasmid DNA with columns without lid



Application High and low copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format Mini prep
Package unit 10 Prep(s), 50 Prep(s), 250 Prep(s), QIAcube 240 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification Centrifugation
Automated use No
Sample material Bacteria, E. coli
Sample amount 1−5 mL (high copy), 6−10 mL (low copy)
Vector size < 25 kbp
Typical yield 25−45 µg
Theoretical binding capacity 60 µg
Typical purity A260/A280 1.8−1.85
Endotoxin level > 50 EU/µg DNA
Elution volume 50 µL
Preparation time 20 min/6 preps
Typical downstream application Automated or manual sequencing, Cloning, hybridization, In-vitro transcription, PCR, Transfection
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Hazardous material Yes





Support Protocols


Agrobacterium tumefaciensPlasmid DNA from yeast

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