Description
Ultrafast plasmid Midi prep due to vacuum processing
Specifications:
| Application | Transfection-grade plasmid DNA isolation |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSnap |
| Format | Snap off column |
| Package unit | 10 Prep(s), 50 Prep(s) |
| Handling | Vacuum, centrifugation for elution |
| Lysate clarification | NucleoSpin Plasmid Filter |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | 50 mL |
| Vector size | < 25 kbp |
| Typical yield | 250 µg |
| Theoretical binding capacity | 1.5 mg |
| Typical purity A260/A280 | 1.8−1.9 |
| Endotoxin level | < 50 EU/µg DNA |
| Elution volume | 200−500 µL |
| Preparation time | 35 min/6 preps |
| Typical downstream application | Transfection of sensitive cells and cell injections |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 27 Months |
| Hazardous material | Yes |