NucleoSpin 96 Plasmid T.-grade

Macherey-NagelSKU: MN-740491.1

Size: (1x96)


A fast way to purify plasmids for transfection



Application High copy plasmid DNA purification
Target Plasmid DNA
CE certified No, research use only
Technology Silica membrane technology and endotoxin reduction technology
Brand NucleoSpin
Format 96-well plate
Package unit 96 Prep(s), 384 Prep(s), 2304 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification NucleoSpin Plasmid Filter Plate
Automated use Yes
Sample material Bacteria, E. coli
Sample amount 1−5 mL
Vector size < 25 kbp
Typical yield 5−20 µg
Theoretical binding capacity 20 µg
Typical purity A260/A280 1.8−1.85
Endotoxin level < 50 EU/µg DNA
Elution volume 100−200 µL
Preparation time 45 min/plate
Typical downstream application Transfection of sensitive cells and cell injections
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Hazardous material Yes





Supplementary Protocol


Application Notes:

Eppendorf epMotion 5075

Hamilton MPE²



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