Mini spin kit for isolation of DNA from stool samples, MN Bead Tubes Type A included
|Application||Isolation of DNA|
|CE certified||No, research use only|
|Technology||Silica membrane technology|
|Package unit||10 Prep(s), 50 Prep(s), 250 Prep(s)|
|Lysate clarification||Centrifugation, NucleoSpin Inhibitor Removal Column|
|Sample amount||180–220 mg|
|Fragment size||200 bp–approx. 50 kbp|
|Typical yield||Depends on sample type, quality, and water content|
|Theoretical binding capacity||50 µg|
|Typical purity A260/A280||1.7–1.9|
|Elution volume||30–100 µL|
|Preparation time||60 min/10 preps|
|Typical downstream application||Microarrays, PCR, Real-time PCR, Southern blotting|
|Storage temperature||15−25 °C|
|Shelf life (from production)||27 Months|
NucleoSpin DNA Stool
- New technology for efficient removal of PCR inhibitors (like polysaccharides or bile salts in stool samples)
- Lysis is supported by mechanical disruption with ceramic beads (MN Bead Tubes Type A)
NucleoSpin DNA Stool procedure
The very efficient, ceramic bead-based lysis procedure achieves unbiased mechanical lysis and makes the NucleoSpin DNA Stool kit ideal for microbiome studies.
Superior DNA yield and inhibition free amplification of the 16S rRNA gene
NucleoSpin DNA Stool quickly and reliably purifies high-quality microbial, host and pathogenic genomic DNA from various stool samples.
High genomic DNA yield, and purity from human stool samples
DNA was isolated from human stool samples with the NucleoSpin DNA Stool kit (MN) and with competitor products (MO, Z, Q). The DNA was extracted according to manufacturer’s protocols, and 5 % of the eluate was subjected to gel electrophoresis. DNA yield, and quality of the samples shown in (A) were assessed by means of UV absorption measurement. The genomic DNA isolated with the NucleoSpin DNA Stool kit showed superior yield, and quality.
Efficient removal of PCR inhibitors from various sample sources
DNA was isolated from human, and animal feces samples with the NucleoSpin DNA Stool kit.5 μL of undiluted eluate served as template for the amplification of a 1.5 kb fragment from the bacterial 16S rRNA gene in an endpoint-PCR (35 cycles). The DNA extracted with the NucleoSpin DNA Stool kit works undiluted in a PCR reaction, indicating the successful removal of PCR inhibitors.
Adamczyk et al. Plant roots increase both decomposition, and stable, organic matter formation in boreal forest soil. Nature Communications, volume 10, 3982 (2019).
Goncalves et al. Microbiota stimulation generates LCMV-specific memory CD8+ T cells in SPF mice, and determines their TCR repertoire during LCMV infection. Molecular Immunology, volume 124, pages 125–141 (2020).