Description
RNA isolation in flexible 96‑well plate format
Specifications:
| Application | Isolation of RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | 96-well plate |
| Package unit | 384 Prep(s) |
| Handling | Centrifugation, vacuum |
| Lysate clarification | Centrifugation, NucleoSpin RNA Filter Strip (optional) |
| Automated use | Yes |
| Application note available with | Corbett, Eppendorf, Hamilton, Tecan |
| Sample material | Animal tissue, Cells, Human tissue, Saliva (collected with Oragene) |
| Sample amount | < 2 x 10⁶ cultured cells, < 20 mg human / animal tissue |
| Fragment size | > 200 nt |
| Typical yield | 20 µg |
| Typical concentration | 50–200 ng/µL |
| Theoretical binding capacity | 100 µg |
| Typical purity A260/A280 | 1.9–2.1 |
| Typical RIN | > 9 (cells), ≥ 7 (tissue) |
| Elution volume | 50–130 µL |
| Preparation time | 70 min/plate |
| Typical downstream application | Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 27 Months |
| Scope of delivery | NucleoSpin RNA Binding Plates (4 x 96), buffers, RNase-free rDNase |
| Hazardous material | Yes |
Application Notes:
Eppendorf epMotion 5075
Tecan Freedom
Tecan Genesis
Corbett X-tractor Gene
Tecan Freedom EVO
Hamilton Genomic STARlet
Hamilton MICROLAB STAR