NucleoSpin 96 RNA Core Kit (4x96)

Macherey-NagelSKU: MN-740466.4



RNA isolation in flexible 96‑well plate format



Application Isolation of RNA
Target RNA
CE certified No, research use only
Technology Silica membrane technology
Brand NucleoSpin
Format 96-well plate
Package unit 384 Prep(s)
Handling Centrifugation, vacuum
Lysate clarification Centrifugation, NucleoSpin RNA Filter Strip (optional)
Automated use Yes
Application note available with Corbett, Eppendorf, Hamilton, Tecan
Sample material Animal tissue, Cells, Human tissue, Saliva (collected with Oragene)
Sample amount < 2 x 10⁶ cultured cells, < 20 mg human / animal tissue
Fragment size > 200 nt
Typical yield 20 µg
Typical concentration 50–200 ng/µL
Theoretical binding capacity 100 µg
Typical purity A260/A280 1.9–2.1
Typical RIN > 9 (cells), ≥ 7 (tissue)
Elution volume 50–130 µL
Preparation time 70 min/plate
Typical downstream application Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays
Storage temperature 15−25 °C
Shelf life (from production) 27 Months
Scope of delivery NucleoSpin RNA Binding Plates (4 x 96), buffers, RNase-free rDNase
Hazardous material Yes





Application Notes:
Eppendorf epMotion 5075
Tecan Freedom
Tecan Genesis
Corbett X-tractor Gene
Tecan Freedom EVO
Hamilton Genomic STARlet


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