Description
Isolation of total DNA from stool samples in 96‑well plate format
Specifications:
Application | Isolation of DNA |
sample amount | 100-200 mg human stool; <100-200 mg animal stool (depending on species of origin and sample quality) |
Target | DNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | 96-well plate |
Package unit | 384 Prep(s), 2304 Prep(s) |
Handling | Vacuum, centrifugation |
Lysate clarification | Vacuum, NucleoSpin Stool Filter Plate |
Automated use | Yes |
Application note available with | Eppendorf |
Sample material | Stool |
Fragment size | 50 bp–approx. 50 kbp |
Typical yield | 3–15 μg (varies by sample and disruption device) |
Theoretical binding capacity | 50 µg |
Typical purity A260/A280 | 1.6–1.8 |
Elution volume | 100−200 µL |
Preparation time | 90 min/48 samples (automated use) |
Typical downstream application | Metagenomics, NGS, qPCR |
Storage temperature | 18–25 °C |
Shelf life (from production) | 24 Months |