NucleoBond Xtra BAC

Macherey-NagelSKU: MN-740436.10

Size: 10


2nd generation of anion exchanger for large construct plasmid DNA



Application Large contruct (P1, BACs, PACs) plasmid purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format BAC prep
Package unit 10 Prep(s), 25 Prep(s)
Handling Gravity flow
Lysate clarification Column filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 250−750 mL
Vector size < 300 kbp
Typical yield 10−150 µg
Theoretical binding capacity 150 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation
Preparation time 75 min/4 preps
Typical downstream application Transfection of sensitive cells and cell injections
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes




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