NucleoBond 96 Xtra EF

Macherey-NagelSKU: MN-740430.1

Size: (1x96)


2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA



Application Endotoxin-free plasmid DNA isolation
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format 96-well plate
Package unit 96 Prep(s), 384 Prep(s)
Handling Gravity flow , vacuum for filtration and precipitation
Lysate clarification NucleoBond Filter Plate
Automated use No
Sample material Bacteria, E. coli
Sample amount 1−5 mL
Vector size < 25 kbp; < 300 kbp (without the NucleoBond Finalizer Plate)
Typical yield 2−4 µg (1.5 mL in 96-well plates), 10−50 µg (5 mL in glass tubes)
Theoretical binding capacity 50 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer Plate
Elution volume 100−200 µL
Preparation time 120 min/plate
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes






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