NucleoBond Xtra Maxi EF

Macherey-NagelSKU: MN-740424.10

Size: 10


2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA


Application Endotoxin-free plasmid DNA isolation
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Maxi prep
Package unit 10 Prep(s), 50 Prep(s)
Handling Gravity flow
Lysate clarification Column filter
Automated use No
Sample material Bacteria, E. coli
Sample amount < 600 mL (high copy), < 1200 mL (low copy)
Vector size < 300 kbp
Typical yield 1000 µg
Theoretical binding capacity 2000 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer Large, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time 90 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes


Gold standard anion exchange technology

Maximum yield and quality. Convince yourself!

  • Our Maxi kits provide Maxi yields above 1 mg
  • Best endotoxin levels in class
  • Convenient and fast processing

Outstanding yields and ultra-pure plasmid DNA

Use NucleoBond Xtra Midi/Maxi EF to get the most out of your plasmid DNA prep! 

Direct comparison of total plasmid yields (pcDNA3.1) grown in LB medium isolated with NucleoBond Xtra Midi EF and Maxi EF as well as different competitor kits according to the respective user manual.

Endotoxins in EU/μg DNA determined after plasmid DNA isolation with NucleoBond Xtra Midi EF and Maxi EF show levels below the FDA limit for vaccines (<0.04 EU/μg DNA).

The direct comparison data show that anion exchange technology has obvious advantages over silica membrane technology in terms of yields and quality. It demonstrates that this technology is still gold standard for purification of high quality plasmid DNA. MN offers with the NucleoBond Xtra Midi/Maxi EF product series excellent kits delivering the quality required for your downstream application in an extremely short processing time.



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