NucleoBond Xtra Midi Plus EF

Macherey-NagelSKU: MN-740422.10

Size: 10


2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA, NucleoBond Finalizer included



Application Endotoxin-free plasmid DNA isolation
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Package unit 10 Prep(s), 50 Prep(s)
Handling Gravity flow
Lysate clarification Column filter
Automated use No
Sample material Bacteria, E. coli
Sample amount < 200 mL (high copy), < 400 mL (low copy)
Vector size 2−50 kbp
Typical yield 500 µg
Theoretical binding capacity 800 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level < 0.1 EU/µg DNA
Isopropanol precipitation NucleoBond Finalizer
Preparation time 45 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes




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