Proven syringe filter for speeding up anion exchange plasmid preparations
|Shelf life||39 Months|
|Application||Concentration and desalting of plasmid eluates|
|CE certified||No, research use only|
|Format||Syringe filter large|
|Package unit||20 Set(s)|
|Sample material||Bacteria, E. coli|
|Sample amount||15 mL DNA eluate|
|Vector size||2−50 kbp|
|Typical recovery||60−90 %|
|Theoretical binding capacity||2000 µg|
|Residual cloride concentration||< 0.3 µg/µl|
|Elution volume||400−1000 µL|
|Preparation time||5 min/prep|
|Typical downstream application||Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells|
|Storage temperature||15−25 °C|
|Shelf life (from production)||24 Months|
Plasmid DNA - Desalting and Concentraction
Following the elution of pure plasmid DNA from the NucleoBond columns, MN provides a range of options for desalting and concentrating the plasmid. In addition to the standard isopropanol precipitation followed by centrifugation we offer the NucleoBond Finalizers for manaul desalting with a syringe as well as centrifuge-compartible NucleoSPin Finisher and vacuum compartible NucleoSnap Finisher for a fast, simple and easy prep.
The NucleoBond Finalizers are designed to concentrate and desalinate plasmid DNA eluates collected using NucleoBond columns after anion-exchange chromatographic DNA purification. The sample is syringe-loaded onto a NucleoBond Finalizer membrane to avoid tedious centrifugation steps.
NucleoSpin and NucleoSnap Finisher
NucleoSpin Finisher are designed for quick concentration and desalting of plasmid eluates obtained by anion-exchange chromatography. Finisher replace tedious and time-consuming isopropanol precipitation. Furthermore, problems like lost DNA pellets or incomplete solubilization of hardly visible precipitates can easily be circumvented.
NucleoMag Desalting Beads
NucleoMag Desalting beads are designed for quick concentration and desalination of plasmid DNA eluates that are obtained be anion exchange chromatographic plasmid purification kits. Plasmid DNA is bound reversibly to NucleoMag Desalting beads followed by ethanolic wash steps. Finally pure plasmid DNA is eluted using a low salt buffer. NucleoMag Desalting beads can be automated on e.g the Andrew+ platform