Description
Customer Testimonial!
"5/5 stars! This kit will purify a 180kb EBV-bacmid. I started with a 400mL culture and purified almost 100ug of bacmid. The final pellet is hard to see because it's so clean - mark the topside of the tube before you spin if you use a fixed angle rotor. It successfully electroporated into my strain of bacteria on the first try. Don't be like me and use other kits - get NucleoBond Xtra before you start any bacmid work and save yourself months of frustration!!"
Kathy - Department of Molecular Biology, University of Toronto
2nd generation of anion exchanger for fast purification of plasmid DNA
Specifications:
| Application | Transfection-grade plasmid DNA isolation |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Package unit | 10 Prep(s), 50 Prep(s), 100 Prep(s) |
| Handling | Gravity flow |
| Lysate clarification | Column filter |
| Automated use | Yes, can be automated on the Andrew+ the pipetting robot |
| Sample material | Bacteria, E. coli |
| Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
| Vector size | < 300 kbp |
| Typical yield | 500 µg |
| Theoretical binding capacity | 800 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | 1−10 EU/µg DNA |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
| Preparation time | 70 min/prep |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 39 Months |
| Hazardous material | Yes |