NucleoBond Xtra Midi

Macherey-NagelSKU: MN-740410.10

Size: 10


Customer Testimonial!

"5/5 stars!  This kit will purify a 180kb EBV-bacmid.  I started with a 400mL culture and purified almost 100ug of  bacmid.  The final pellet is hard to see because it's so clean - mark the topside of the tube before you spin if you use a fixed angle rotor.  It successfully electroporated into my strain of bacteria on the first try.  Don't be like me and use other kits - get NucleoBond Xtra before you start any bacmid work and save yourself months of frustration!!"

Kathy - Department of Molecular Biology, University of Toronto

2nd generation of anion exchanger for fast purification of plasmid DNA



Application Transfection-grade plasmid DNA isolation
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Package unit 10 Prep(s), 50 Prep(s), 100 Prep(s)
Handling Gravity flow
Lysate clarification Column filter
Automated use Yes, can be automated on the Andrew+ the pipetting robot
Sample material Bacteria, E. coli
Sample amount < 200 mL (high copy), < 400 mL (low copy)
Vector size < 300 kbp
Typical yield 500 µg
Theoretical binding capacity 800 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time 70 min/prep
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes





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