NucleoZOL (200 mL)

Macherey-NagelSKU: MN-740404.200



Total RNA extraction from all kinds of sample



Application Isolation of total RNA
Target RNA
CE certified No, research use only
Technology One phase extraction
Brand NucleoZOL
Format Reagent
Package unit 200 mL
Handling Centrifugation
Lysate clarification Centrifugation
Automated use No
Sample material Animal tissue, Bacteria, Cells, Human tissue, Plant, Viral fluid, Yeast
Sample amount < 2 x 10⁶ cultured cells, < 2 x 10⁶ bacterial cells, < 2 x 10⁶ yeast cells, < 100 mg human /animal / plant tissue, < 0.4 mL viral fluid
Fragment size Total RNA > 10 nt (small RNA 10-200 nt, large RNA > 200 nt)
Typical yield 1–8 µg total RNA/mg sample
Typical purity A260/A280 1.8–2.1
Typical RIN > 9
Elution volume Flexible
Preparation time < 1 h
Typical downstream application Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Scope of delivery NucleoSpin RNA Columns, Collection Tubes, buffers
Hazardous material Yes






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