Description
Large scale isolation of RNA from whole blood
Specifications:
| Application | Isolation of RNA |
| Target | RNA |
| CE certified | No, research use only |
| Technology | Silica membrane technology |
| Brand | NucleoSpin |
| Format | Midi prep |
| Package unit | 20 Prep(s) |
| Handling | Centrifugation |
| Automated use | No |
| Sample material | Blood |
| Sample amount | 400–1300 µL |
| Fragment size | > 200 nt |
| Typical yield | 4–26 µg (1300 µL whole blood) |
| Theoretical binding capacity | 700 µg |
| Typical purity A260/A280 | 1.9–2.1 |
| Elution volume | 200–400 µL |
| Preparation time | 75 min/6 preps |
| Typical downstream application | Array technology, Northern blotting, Primer extension, qRT−PCR, RNA-Seq, RNase protection assays |
| Storage temperature | 15−25 °C |
| Shelf life (from production) | 24 Months |
| Scope of delivery | NucleoSpin RNA Blood Midi Columns, Collection Tubes, buffers, RNase-free rDNase, Liquid Proteinase K |
| Hazardous material | Yes |