NucleoBond HMW DNA (20)

Macherey-NagelSKU: MN-740160.20



Anion exchange chromatography for purification of high integrity DNA from different sample materials



Shelf life 39 Months
Application Isolation of DNA
Target DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format Midi prep
Package unit 2 Prep(s), 20 Prep(s)
Handling Gravity flow
Automated use No
Sample material Animal tissue, Bacteria, Blood, Body fluids, Cells, Human tissue, Plant, Yeast
Sample amount < 300 mg human/animal tissue, < 10⁷ cultured cells, < 1.5 g plant leaves, < 30 mg bacteria/yeast (ground under liquid nitrogen), < 300 mg bacteria/yeast (lysed enzymatically or by bead beating), < 2 mL liquid sample
Fragment size 2 kbp–approx. 150 kbp (enzymatic lysis)/50 kbp (mechanical lysis)
Typical yield Depending on amount and quality of sample
Theoretical binding capacity 600 µg
Typical purity A260/A280 1.8–1.9
Elution volume 50–250 µL
Preparation time 2 h/12 preps (incl. 30 min lysis)
Typical downstream application Long-read sequencing, Next Generation Sequencing
Storage temperature 15−25 °C
Shelf life (from production) 39 Months
Hazardous material Yes





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