Description
Isolation of RNA from challenging plant material and fungi
Specifications:
Application | Isolation of RNA |
Target | RNA |
CE certified | No, research use only |
Technology | Silica membrane technology |
Brand | NucleoSpin |
Format | Mini prep |
Package unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
Handling | Centrifugation, vacuum |
Lysate clarification | Centrifugation, NucleoSpin Filter |
Automated use | No |
Sample material | Fungi, Plant |
Sample amount | < 500 mg |
Fragment size | > 200 nt |
Typical yield | 20–70 µg |
Theoretical binding capacity | 200 µg |
Typical purity A260/A280 | 1.9–2.1 |
Typical purity A260/A230 | Approx. 2 |
Typical RIN | 7−9 |
Elution volume | 50 µL |
Preparation time | 25 min/6 preps |
Typical downstream application | Gene expression profiling, Northern blotting, Primer extension, Real−time RT−PCR |
Storage temperature | 15−25 °C |
Shelf life (from production) | 24 Months |
Hazardous material | Yes |